Catalogue No. Quantity/Specifications
Cat # BB-AB0119 100 µl (100 µg)


Technology

Anti-β Tubulin Antibody

PDF DOWNLOAD

Anti-β Tubulin Antibody

Product type: Primary Antibody
Clonality:       Polyclonal
Source:          Rabbit
Immunogen: Full length Human β-tubulin protein expressed in E. coli.
Description: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. Microtubules are involved in a wide variety of cellular activities ranging from mitosis/meiosis, beating of cilia and flagella, transport of membrane vesicles to cell movement and the maintenance of cell shape. α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species.
Purification: Purified against β-tubulin protein (Human full length) affinity chromatography.
Specificity: β-tubulin polyclonal antibody detects human full length β-tubulin proteins expressed in eukaryotic cells.
Recommended Dilutions:
Western Blotting 1:2,000 to 5,000
ELISA                    1:10,000

Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubation and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.