Catalogue No. Quantity/Specifications
Cat # BB-AB0120 100 µl (100 µg)


Technology

Anti-γ Tubulin Antibody

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Anti-γ Tubulin Antibody

Product type:  Primary Antibody
Clonality:         Polyclonal
Source:            Rabbit
Immunogen: Recombinant mouse gamma tubulin protein expressed in E. coli.
Description: Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. As a critical part of the microtubule-organizing center (MTOC), the third member of the tubulin superfamily, γ-tubulin, is required for microtubule nucleation as well as centrosome duplication and spindle assembly. Gamma tubulin is a ~49 KDa mammalian protein. Anti-γ tubulin antibody is primarily used to test purity of cytoplasmic cell fractionation and loading control. This antibody is produced in rabbit by repeated injections of Immunogen.

Purification: Purified against gamma tubulin protein affinity chromatography.
Specificity: This antibody recognizes endogenous levels of total γ-tubulin protein.
Recommended Dilutions:
Western Blotting 1:2,000 to 5,000
ELISA                   1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.