Catalogue No. Quantity/Specifications
Cat # BB-AB0225 100 µl (100 µg)


Technology

Anti-ATG5 Antibody

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Anti-ATG5 Antibody

Product type:  Primary Antibody
Clonality:         Polyclonal
Source:            Rabbit
Immunogen:  Recombinant human ATG5 protein expressed in E. coli.
Other name:  APG5L
Description:  ATG5 (autophagy protein 5, also known as APG5-like, apoptosis-specific protein, APG5L, and ASP) belongs to ATG5 family and is an essential protein for autophagy. It is expressed ubiquitously and the expression increases dramatically in apoptotic cells. ATG5 is localized in cytoplasm (can co-localize with non muscle actin) and is induced upon apoptotic stimuli. ATG5-ATG12 conjugate forms a complex with several units of ATG16 and this complex inhibits ATG5-TECPR1 direct interactions. ATG5 play an important role in the apoptotic process, within the modified cytoskeleton, and its expression is a relatively late event in apoptotic process, occurring downstream of caspase activity.
Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity: This antibody detects ATG5 protein in eukaryotic cell extracts and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting 1:5,000
ELISA                    1:1,000

Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.