Catalogue No. Quantity/Specifications
Cat # BB-AB0180 100 µl (100 µg)


Technology

Anti-ATPS1α Antibody

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Anti-ATPS1α Antibody

Product type: Primary Antibody
Clonality:        Polyclonal
Source:           Rabbit
Immunogen: Recombinant full length human ATPS1α expressed in E. coli.
Description: Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. The catalytic portion of mitochondrial ATP synthase is the assembly of 5 different subunits (alpha, beta, gamma, delta, and epsilon). ATPS1α protein migrates as a ~54 KDa band in SDS-PAGE.

Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity: This antibody detects ATPS1-α protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.

Recommended Dilutions:
Western Blotting        1:5,000
ELISA                           1:1,000

Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.