Catalogue No. Quantity/Specifications
Cat # BB-AB0230 100 µl (100 µg)


Technology

Anti-Bcl2 Antibody

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Anti-Bcl2 Antibody

Product type: Primary Antibody
Clonality:        Polyclonal
Source:           Rabbit
Immunogen: Recombinant N-terminal domain of human Bcl2 expressed in E. coli.
Description:  Members of the Bcl-2 Family (including Bcl-2, Bcl-XL and Bax) play an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, Bax is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and hetero dimerization of different isoforms of Bax and Bcl-2. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3′ UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 KDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 KDa).
Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity: This antibody detects Bcl2 protein in human and mouse & Rat cell extracts.
Recommended Dilutions:
Western Blotting 1:1,000
ELISA                    1:5,000 to 1:10,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubation and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.