Product type:   Primary Antibody
Clonality:          Polyclonal
Source:             Rabbit
Immunogen:    KLH-coupled human Caspase 3 synthetic peptide.
Description:Caspase-3 (CPP-32, Apoptain,SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position.
Purification: Purified against peptide affinity chromatography.
Specificity:  This antibody detects Caspase3 in human, Mice cell line extract.
Recommended Dilutions:
Western Blotting 1:2000

Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.