Product type: Primary Antibody
Clonality:        Polyclonal
Source:           Rabbit
Immunogen:  KLH-coupled human Caspase 8 synthetic peptide.
Description:  Caspase 8 is considered to be an initiator caspase. It exists in the cell as an inactive 55 KDa proenzyme form. Caspase 8 activates downstream caspases viz; caspases 3, 6, and 7 that cleave key cellular substrates and lead to apoptotic death of the cells, thus play a crucial role in the programmed cell death.
Purification: Purified against peptide affinity chromatography.
Specificity: This antibody detects Caspase 8 in human cell line extract.
Recommended Dilutions:
Western Blotting: 1: 2,000

Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.