Catalogue No. Quantity/Specifications
Cat # BB-AB0135 100 µl (100 µg)


Technology

Anti-Erk2/MAPK1 Antibody

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Anti-Erk2/MAPK1 Antibody

Product type: Primary Antibody
Clonality:        Polyclonal
Source:           Rabbit
Immunogen: Full length mouse Erk2 protein (42 KDa) expressed in E. coli.
Description: Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42MAPK (ERK1/2) signalling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines.
Anti- Erk2 antibody can be used for detection of Erk2protein by immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen.

Purification: Purified against Erk2 protein affinity chromatography.
Specificity: This antibody detects Erk2protein in eukaryotic cell (human, mice) extract and recombinant Erk2proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting 1:5,000
ELISA 1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.