Catalogue No. Quantity/Specifications
Cat # BB-AB0065 100 µl (100 µg)


Technology

Anti-GFP Antibody

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Anti-GFP Antibody

Product type: Primary Antibody
Clonality:        Polyclonal
Source:           Rabbit
Immunogen: Green-fluorescent protein (GFP).
Description: The green-fluorescent protein (GFP) (27 KDa) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission
spectrum that peaks at 509 nm. Anti-GFP antibody is the tool for detection of GFP-tag proteins by different immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen.
Purification: Purified against protein affinity chromatography.
Specificity: Anti-GFP polyclonal antibody detects GFP-fussed recombinant proteins expressed in eukaryotic and prokaryotic cells.
Recommended Dilutions:
Western Blotting    1:10,000
ELISA                       1:2,000

Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.