Catalogue No. Quantity/Specifications
Cat # BBGF 002A 0.1 ml Packed Bead Volume
Cat # BBGF 002B 0.25 ml Packed Bead Volume


Technology

Anti-GFP IgG Agarose Beads

PDF DOWNLOAD

Anti-GFP IgG Agarose Beads

The green-fluorescent protein (GFP) (27 KDa) functions as a bioluminescence energy transfer acceptor in the Jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. Anti-GFP antibody is the tool for detection of GFP-tag proteins by different immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen.
Anti-GFP IgG was covalently coupled to solid support Agarose beads to prepare affinity purification matrix to purify GFP or GFP tagged proteins. Approximately 2 mg of anti-GFP antibody has been coupled with Agarose beads and the affinity matrix has purification capacity of ∼1 mg free green fluorescence protein from crude bacterial cell

TECHNICAL SPECIFICATIONS
BEAD GEOMETRY & SIZE : Spherical, ∼ 50 – 150 μm diameter
CROSSLINKED : Yes
BEAD AGAROSE % : 6%
ACTIVATING GROUP : Carbonyl
COUPLED ANTIBODY : ∼2 mg antibody/ml pack bead
PURIFICATION CAPACITY : ∼1 mg GFP protein/ml pack bead
MATRIX STABILITY : Stable in all commonly used reagents
STORAGE SOLUTION : 20% aqueous ethanol
STORAGE TEMPERATURE : 4°C to 8°C. DO NOT FREEZE.