Catalogue No. Quantity/Specifications
Cat # BB-AB0220 100 µl (100 µg)


Technology

Anti-HSF1 Antibody

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Anti-HSF1 Antibody

Product type:  Primary Antibody
Clonality:         Polyclonal
Source:            Rabbit
Immunogen:   Recombinant Full length human HSF1 expressed in E. coli.
Other name:   Heat shock factor 1
Description:    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli; HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity: This antibody detects HSF1 protein in human and mouse cell extracts.
Recommended Dilutions:
Western Blotting      1:2,000 to 5,000
ELISA                      1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.