Catalogue No. Quantity/Specifications
Cat # BB-AB0209 100 µl (100 µg)


Technology

Anti-HSP60 Antibody

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Anti-HSP60 Antibody

Product type: Primary Antibody
Clonality:        Polyclonal
Source:           Rabbit
Immunogen:   Recombinant Full length human Hsp60 expressed in E. coli.
Description:    Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP60, a ~60 KDa protein, is the human homologue of HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred as chaperonin-60, P1 & mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is a constitutively expressed mitochondrial protein that shares many characteristics with HSP70 including high abundance, induction by environmental stress, ATPase activity and a critical role in protein assembly and folding.
Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity: This antibody detects Hsp60 protein in human and mouse cell extracts.
Recommended Dilutions:
Western Blotting 1:5,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.