Product type: Primary Antibody

Clonality:        Polyclonal

Source:            Rabbit

Immunogen: Recombinant N-terminal domain of human His-Hsp70 expressed in E. coli.

Other name: Hsp70

Description: Members of Hsp70 family proteins are over expressed under stress such as heat shock and oxidative conditions. Hsp70 prevents its substrate to form aggregates.

Purification: Purified by affinity chromatography using protein A/G affinity matrix.

Specificity: This antibody detects Hsp70 protein in human and mouse cell extracts.

Recommended Dilutions:                                               

Western Blotting               1:5,000
ELISA                                  1:1,000

Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.