Catalogue No. Quantity/Specifications
Cat # BB-AB0155 100 µl (100 µg)


Technology

Anti-Human IL-1β Antibody

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Anti-Human IL-1β Antibody

Product type:   Primary Antibody
Clonality:         Polyclonal
Source:            Rabbit
Immunogen:   Recombinant full length human IL-1β expressed in E. coli.
Description: Interleukin 1b is a cytokine critical for inflammatory and immune responses. It is 17 KDa in length. IL-1β has also been implicated in several pathological conditions including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis.
Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity:   This antibody detects IL-1β protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting 1:2,000
ELISA                    1:5,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.