|Cat # BB-AB0170||100 µl (100 µg)|
Anti-Human TNFα AntibodyPDF DOWNLOAD
Product type: Primary Antibody
Immunogen: Recombinant human TNF-α expressed in E. coli.
Description: TNF-α is a Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is secreted by many cell types but primarily by macrophages. TNF-α can induce cell proliferation, inflammation and cell death. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion.
Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity: This antibody detects TNF-α protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Western Blotting 1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.