Catalogue No. Quantity/Specifications
Cat # BB-AB0095 100 µl (100 µg)


Technology

Anti-IKK2/β Antibody

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Anti-IKK2/β Antibody

Product type:   Primary Antibody
Clonality:          Polyclonal
Source:             Rabbit
Immunogen:    Recombinant IKKβ protein (11 KDa) (residues 678-756) expressed in E. coli.
Description: The cytosolic NFκB/Rel transcription factors are in inactive state, complexed with the inhibitory IκB proteins. Phosphorylation-induced, proteasome-mediated degradation of IκB triggers their activation. The key regulatory step in this pathway involves activation of IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase. IKKγ serves as the regulatory subunit. Phosphorylation of serines 177 and 181 in the activation loop of IKKβ (176 and 180 in IKKα) causes conformational changes resulting in activation of IKK. There is strong biochemical and genetic evidence that the IKK complex, which consists of two catalytic subunits, IKK alpha and IKK beta, and a regulatory subunit, IKK gamma, is the master regulator of NF-kappa B-mediated innate immune and inflammatory responses. In the absence of IKK gamma, which normally connects IKK to upstream activators, no IKK or NF-kappa B activation can occur. Surprisingly, however, of the two catalytic subunits, only IKK beta is essential for NF-kappa B activation in response to proinflammatory stimuli. The second catalytic subunit, IKK alpha, plays a critical role in developmental processes, in particular formation and differentiation of the epidermis. This antibody is produced in rabbit by repeated injections of Immunogen.

Purification: Purified against IKKβ protein (residues 678-756) affinity chromatography.
Specificity: This antibody detects IKKβ protein (~85 KDa) in eukaryotic cell extracts and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting   1:2000
ELISA                      1:1000
IP                             1:100
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.