Product type:  Primary Antibody
Clonality:         Polyclonal
Source:            Rabbit
Immunogen:   Recombinant full length mouse IL-6 expressed in E. coli.
Description:    Interleukin 6 is a cytokine relevant to many inflammatory diseases and many cancers.Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length that plays critical roles in host defense, immune response and hematopoiesis. Like IL-1β and TNF-α, IL-6 acts as endogenous pyrogen, regulating the fever response to bacterial invasion.
Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity: This antibody detects IL-6 protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting 1:2,000
ELISA                    1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubation and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.