Catalogue No. Quantity/Specifications
Cat # BB-AB0175 100 µl (100 µg)


Technology

Anti-mTNF-α Antibody

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Anti-mTNF-α Antibody

Product type: Primary Antibody
Clonality:       Polyclonal
Source:          Rabbit
Immunogen: Recombinant full length mouse TNF-α expressed in E. coli.
Description: TNF-α is a Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is secreted by many cell types but primarily by macrophages. TNF-α can induce cell proliferation, inflammation and cell death. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion.

Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity:  This antibody detects TNF-α protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting       1:2,000
ELISA                          1:5,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubation and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.