Catalogue No. Quantity/Specifications
Cat # BB-AB0115 100 µl (100 µg)


Technology

Anti-NIK Antibody

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Anti-NIK Antibody

Product type: Primary Antibody
Clonality: Polyclonal
Source: Rabbit
Immunogen: Synthetic VEEYLVHALQGSVSC peptide sequence of human NIK protein coupled with KLH.

Description: Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. The p50 and p52 products form dimeric complexes with Rel proteins, which are then able to bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by its inhibitory proteins, the IκB’s. NF-κB-activating agents can induce the phosphorylation of IκB’s, targeting them for rapid degradation through a ubiquitin-proteasome pathway, releasing NF-κB to enter the nucleus, where it regulates gene expression. Processing of NF-κB2 p100 is regulated by NIK and IKK1 (IKKα), which triggers the phosphorylation and processing to p52, which can then undergo nuclear translocation.
Activation of NF-kB can be controlled by NF-kB-inducing kinase (NIK), a member of the MAP3K family that was originally identified as a TRAF2-interacting protein and thereby coupled to receptor activation. NIK forms a complex with and phosphorylates IKK1 and IKK2, subsequently leading to the phosphorylation of IkB and translocation of NF-kB to the nucleus.
Purification: Purified against peptide affinity chromatography.
Specificity: NIK Antibody detects endogenous levels of total NIK protein.
Recommended Dilutions:
Western Blotting   1:5,000
ELISA                      1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.