|Cat # BB-AB0125||100 µl (100 µg)|
Product type: Primary Antibody
Immunogen: Human p100 (residues 436-899) protein expressed in E. coli.
Description: p100 is 100 KDa protein which serves as the precursor of NF-kB family protein p52/NF-kB2. Unprocessed p100 functions as an inhibitor of NF-kB proteins including its processed product p52.
Anti-p100 antibody can be used for detection of only unprocessed p100and not its processed product by immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen.
Purification: Purified against p100 (residues 436-899) protein affinity chromatography.
Specificity: This antibody detects P100 protein in eukaryotic cell (human, mice) extract and recombinant p100 proteins expressed in prokaryotic cells.
Western Blotting 1:1000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.