Catalogue No. | Quantity/Specifications |
---|---|
Cat # BB-AB0185 | 100 µl (100 µg) |
Anti-p27 Antibody
Product type: Primary Antibody
Clonality: Polyclonal
Source: Rabbit
Immunogen: Recombinant full lengthhumanp27 expressed in E. coli.
Other name: Kip1
Description: Cell cycle dependent protein kinases (CDKs) in association with cyclins control cell cycle. A class of factors described as ‘mitotic inhibitors’ negatively regulates cell cycle progression. p27 is one of these cell cycle inhibitors. p27 is a 158-residue protein migrates as a ~27 KDa band in SDS-PAGE.
Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity: This antibody detects p27 protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting 1:2,000
ELISA 1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.