Catalogue No. Quantity/Specifications
Cat # BB-AB0185 100 µl (100 µg)


Technology

Anti-p27 Antibody

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Anti-p27 Antibody

Product type:  Primary Antibody
Clonality:         Polyclonal
Source:            Rabbit
Immunogen:   Recombinant full lengthhumanp27 expressed in E. coli.
Other name:   Kip1

Description:   Cell cycle dependent protein kinases (CDKs) in association with cyclins control cell cycle. A class of factors described as ‘mitotic inhibitors’ negatively regulates cell cycle progression. p27 is one of these cell cycle inhibitors. p27 is a 158-residue protein migrates as a ~27 KDa band in SDS-PAGE.
Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity: This antibody detects p27 protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting   1:2,000
ELISA                      1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.