Product type:  Primary Antibody
Clonality:         Polyclonal
Source:            Rabbit
Immunogen:   Human p62 protein expressed in E. coli.
Description:    p62, also known as Sequestosome 1 (SQSTM1, p62) is an ubiquitin binding protein that plays a role in cell signalling, oxidative stress and autophagy.
Anti-P62 antibody can be used for detection of P62protein by immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen.

Purification: Purified against P62 protein affinity chromatography.
Specificity: This antibody detects P62 protein in eukaryotic cell (human, mice) extract and recombinant p62 proteins expressed in prokaryotic cells.

Recommended Dilutions:
Western Blotting               1:2,000

ELISA                                  1:1,000

Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.