Product type:   Primary Antibody
Clonality:          Polyclonal
Source:             Rabbit
Immunogen:     KLH-coupled human PARP 1 synthetic peptide.
Description: PARP1 has a role in single-stranded DNA (ssDNA) breaks repair. Knocking down of intracellular PARP1 levels or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks.
Purification: Purified against peptide affinity chromatography.
Specificity: This antibody detects PARP1 in mouse, human cell line.
Recommended Dilutions:
Western Blotting 1:1000

Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.