Catalogue No. Quantity/Specifications
Cat # BB-AB0190 100 µl (100 µg)


Technology

Anti-REGβ Antibody

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Anti-REGβ Antibody

Product type: Primary Antibody
Clonality:        Polyclonal
Source:            Rabbit
Immunogen:   Recombinant full length human REGβ expressed in E. coli.
Other name: PA28β, 11Sβ
Description: REGβ is an activator of 20S proteasome and is responsible for ATP-independent degradation of unfolded proteins or peptides.     REGβ forms hetero heptamer with REGα subunit.  The heptamer ring sits atop the α-ring of the 20S proteasome. REGβ monomer is 28 KDa in MW.
Purification: Purified by affinity chromatography using protein A/G affinity matrix.
Specificity: This antibody detects REGβ protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions: 
Western Blotting               1:20,000
ELISA                                  1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk.  All incubation and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.