Catalogue No. Quantity/Specifications
Cat # BB-AB0195 100 µl (100 µg)


Technology

Anti-SRPK1 Antibody

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Anti-SRPK1 Antibody

Product type:  Primary Antibody
Clonality:        Polyclonal
Source:            Rabbit
Immunogen:   Recombinant truncated human SR protein kinase 1 (SRPK1) expressed in E. coli.
Other name:   SRSF protein kinase
Description:   SRPK1 is a 655 residues long protein kinase which specifically phosphorylates serine residues of Ser-Arg (SR) protein splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. It migrates aberrantly in SDS-PAGE as a ~100 KDa protein.

Purification: Purified by affinity chromatography using a SRSF1 affinity matrix.
Specificity: This antibody detects SRPK1 protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting    1:2,000
ELISA                       1:1,000

Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.