Catalogue No. Quantity/Specifications
Cat # BB-AB0140 100 µl (100 µg)


Technology

Anti-SRSF1(ASF) Antibody

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Anti-SRSF1(ASF) Antibody

Product type:  Primary Antibody
Clonality:         Polyclonal
Source:            Rabbit
Immunogen:  Recombinant truncated human SRSF1expressed in E. coli.
Other name:   ASF, SF2
Description:    SRSF1 is a SR (Ser-Arg) family of proteins, which contains the RNA binding domain in its N-terminus and RS domain in the C-terminus. It is a splicing factor which modulates splicing by binding to exonic splicing enhancers (ESE) present in pre-mRNA substrates. SRSF1 migrates approximately as a 35 KDa protein in SDS-PAGE.

Purification: Purified by affinity chromatography using a SRSF1 affinity matrix.
Specificity: This antibody detects SRSF1 protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting             1:1,000
ELISA                                1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.