Catalogue No. Quantity/Specifications
Cat # BB-AB0270 100 µl (100 µg)


Technology

Anti-Streptavidin Antibody

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Anti-Streptavidin Antibody

Product type:  Primary Antibody
Clonality:         Polyclonal
Source:            Rabbit
Immunogen:  Recombinant full length Streptavidin protein from Streptomyces avidinii
Description:   Streptavidin is a tetrameric protein purified from Streptomyces avidinii. The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is used extensively and widely in numerous molecular biological protocols. The interaction of sterpatavidin-botin is resistance to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH.
Purification: Purified against recombinant full length Streptavidin protein by affinity chromatography.
Specificity: This antibody detects Streptavidin tagged protein and recombinant Streptavidin protein expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting   1:2,000
ELISA                      1:5,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.