|Cat # BB-AB0270||100 µl (100 µg)|
Anti-Streptavidin AntibodyPDF DOWNLOAD
Product type: Primary Antibody
Immunogen: Recombinant full length Streptavidin protein from Streptomyces avidinii
Description: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is used extensively and widely in numerous molecular biological protocols. The interaction of sterpatavidin-botin is resistance to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH.
Purification: Purified against recombinant full length Streptavidin protein by affinity chromatography.
Specificity: This antibody detects Streptavidin tagged protein and recombinant Streptavidin protein expressed in prokaryotic cells.
Western Blotting 1:2,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.