Catalogue No. Quantity/Specifications
Cat # BB-AB0200 100 µl (100 µg)


Technology

Anti-U1-70K Antibody

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Anti-U1-70K Antibody

Product type:  Primary Antibody
Clonality:         Polyclonal
Source:            Rabbit
Immunogen:  Recombinant human U1-70K (residues 1-215) expressed in E. coli.
Other name:  SNRNP70

Description:  U1-70K is a component of U1 snRNP involved in pre-mRNA splicing which binds to the loop 1 region of U1 snRNA. U1 snRNP is involved in the pre-spliceosome formation. U1-70K has RNA binding domain in its N-terminus and RS (arginine-serine)-like domain in the C-terminus. U1-70K is 436-residue long protein. Although the approximate MW of this protein ~50 KDa, it aberrantly migrates as a 70 KDa protein in SDS-PAGE.
Purification: Purified by affinity chromatography using a U170K affinity matrix.
Specificity: This antibody detects U1-70K protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting   1:2,000
ELISA                      1:1,000

Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubation and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.