Catalogue No. Quantity/Specifications
Cat # BB-AB0205 100 µl (100 µg)


Technology

Anti-U2AF65 Antibody

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Anti-U2AF65 Antibody

Product type:  Primary Antibody
Clonality:         Polyclonal
Source:            Rabbit
Immunogen:  Recombinant truncated human U2AF65 expressed in E. coli.
Other name:  U2AF2
Description:   U2 auxiliary factor 65 (U2AF65) is the large subunit of the splicing factor, U2AF. It is a non-snRNP protein binds to the poly-pyrimidine tract (PPT) located near the branch site (BS) of pre-mRNA substrate. U2AF65 is required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF65 is a 475-residue long protein migrates as a 65 KDa protein in SDS-PAGE.
Purification: Purified by affinity chromatography using a U2AF65 affinity matrix.
Specificity: This antibody detects U2AF65 protein in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Recommended Dilutions:
Western Blotting   1:2,000
ELISA                      1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.