Immobilized metal ion affinity chromatography (IMAC) is the most widely used purification technique. It is based on the interaction between certain protein residues (histidines, cysteines), with transition metal cations forming chelates. These transition metal ions bind to the agarose beads through a chemical reaction, giving the agarose an activation state.

Nitrilotriacetic acid (NTA)-crosslinked-agarose resin consists of NTA groups covalently immobilized on agarose beads by stable ether linkages via a spacer arm. NTA is a tetradentate chelating agent forms octahedral coordination complex with divalent transition metal cations (Co2 ) along with two water molecules occupied in cis manner. This ready to use resin, after loading with a divalent transition metal ion (Co2 ) is ideal for rapid purifications of His-tagged proteins. In comparison with other chelating resins such as iminodiacetic acid (IDA)-agarose, the NTA has two sites (cis) available for the interaction with imidazole of His-tagged proteins, instead of the three in IDA, and so have lesser probability of nonspecific binding. NTA resins are usually more easily regenerated, allowing a better elution of the fused proteins bound with smaller concentrations of imidazole.

Though Ni-resin is being widely used for His-tag recombinant protein purification but in some cases Co-resins is preferable due to its higher specificity. Where purity is of prior importance over quantity, it is recommended to use Co-resins for better result. Product is supplied as 50% suspension of Co-NTA Agarose resin in 20% aqueous ethanol; the settled bead volume is measured in ml, when supplied.
> BioBharati Co-NTA resins can withstand autoclaving at 120°C for 20 min without any significant loss of binding efficiency.

TECHNICAL SPECIFICATIONS
BEAD GEOMETRY & SIZE :  Spherical, Standard
                                              ∼ 50-150 μm diameters
CROSS-LINKED :                    Yes
AGAROSE % :                          4%
ACTIVATING GROUP :           Epoxy
MATRIX STABILITY :             Stable in all commonly used reagents
STORAGE SOLUTION :          20 % Ethanol
STORAGE TEMPERATURE :  4°C to 8°C.  DO NOT FREEZE.
For Research use only – Not for use in diagnostics procedures.