Glutathione Agarose Beads
Glutathione Agarose bead is designed for high capacity single-step affinity purification of Glutathione S-Transferase (GST) tagged fusion proteins. This product consists of reduced glutathione attached to epoxy activated 4% cross-linked beaded agarose through the sulfur atom with a 12 atom (10 carbon) spacer arm. This 12 atom spacer arm between bead and the ligand allows strong affinity interaction between glutathione & GST part of fusion protein and minimizes steric inhibition.
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing, and highly selective purification of glutathione binding enzymes such as GST, glutathione peroxidase, and glyoxalase I. GST-tagged fusion proteins or other glutathione-dependent proteins can be purified directly from bacterial lysates using this bead with very high purity. Also the mild non-denaturing elution condition preserves protein antigenicity.
BEAD GEOMETRY & SIZE : Spherical, ∼ 50 – 150 μm diameter
CROSS-LINKED : Yes
BEAD AGAROSE % : 4%
ACTIVATING GROUP : Epoxy
SPACER ARM : 12 atom spacer arm (10 carbons)
PURIFICATION CAPACITY : ∼30 mg GST tagged fusion protein/ml pack bead
MATRIX STABILITY : Stable in all commonly used reagents
STORAGE SOLUTION : 20% aqueous ethanol
STORAGE TEMPERATURE : 4°C to 8°C. DO NOT FREEZE.