Catalogue No. Quantity/Specifications
Cat# BB-E0040 20,000 U (100 µl) 100 Reactions


Technology

MuLV Reverse Transcriptase

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MuLV Reverse Transcriptase

Description: Murine Leukemia Virus Reverse Transcriptase (MuLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (upto 5 Kbp). The enzyme is isolated from E.coli expressing a portion of pol gene of MuLV on a plasmid. The RNase H activity of MuLV RT is weaker than the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Reagents supplied:
• MuLV RT (200 U/µl), 100 µl
• 5x RT Buffer (250 mM Tris-HCl, pH 8.4; 375 mM KCl; 15 mM MgCl2), 400 µl

Storage Buffer: 20mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol (v/v).
Storage Instruction: -20°C.