Objectives:
• To perform PCR amplification of specific target sequence from template DNA.
• To analyzed the amplified product by Agarose gel electrophoresis.
Principle:
PCR-polymerase chain reaction is an in vitro method of enzymatic synthesis of specific DNA sequence, developed by Kary Mullis in 1983. It is a very simple technique for characterizing, analyzing & synthesizing any specific DNA or RNA from any source.
Materials provided: (for 10 reactions)
The list bellow provides the information about the materials supplied in the kit.
Materials Quantity Storage Condition
Taq DNA polymerase 10 µl -20°C
10X Taq Buffer 60 µl -20°C
Template DNA 10 µl -20°C
Forward Primer 10 µl -20°C
Reverse Primer 10 µl -20°C
Nuclease free water 1 ml -20°C
1Kbp DNA ladder 50 µl -20°C
dNTP mix 10 µl -20°C
6 X DNA Loading Dye 100 µl -20°C
Agarose 1 gm RT
50 X TAE 15 ml 4°C
PCR Tubes 10 Nos. RT
Ethidium bromide (EtBr) 50 ul RT