Objectives:
• To perform PCR amplification of specific target sequence from template DNA.
• To analyzed the amplified product by Agarose gel electrophoresis.

Principle:
PCR-polymerase chain reaction is an in vitro method of enzymatic synthesis of specific DNA sequence, developed by Kary Mullis in 1983. It is a very simple technique for characterizing, analyzing & synthesizing any specific DNA or RNA from any source.

Materials provided: (for 10 reactions)
The list bellow provides the information about the materials supplied in the kit.

Materials                         Quantity         Storage Condition
Taq DNA polymerase      10 µl             -20°C
10X Taq Buffer                 60 µl             -20°C
Template DNA                 10 µl             -20°C
Forward Primer                10 µl            -20°C
Reverse Primer                10 µl             -20°C
Nuclease free water         1 ml            -20°C
1Kbp DNA ladder            50 µl            -20°C
dNTP mix                         10 µl            -20°C
6 X DNA Loading Dye   100 µl           -20°C
Agarose                             1  gm           RT
50 X TAE                          15 ml             4°C
PCR Tubes                       10 Nos.         RT
Ethidium bromide (EtBr) 50 ul            RT