Objectives:
• To perform PCR amplification of specific target sequence from template DNA.
• To analyzed the amplified product by Agarose gel electrophoresis.

Principle:
PCR-polymerase chain reaction is an in vitro method of enzymatic synthesis of specific DNA sequence, developed by Kary Mullis in 1983. It is a very simple technique for characterizing, analyzing & synthesizing any specific DNA or RNA from any source.

Materials                      Quantity             Storage Condition
Taq DNA polymerase      5µl                    -20°C
10X Taq Buffer                 50 µl                 -20°C
Template DNA                  5µl                    -20°C
Forward Primer                5µl                    -20°C
Reverse Primer                5µl                    -20°C
Nuclease free water       200 µl               -20°C
1kb DNA ladder                25 µl                -20°C
dNTP mix                          10 µl                -20°C
6X DNA Loading Dye       25 µl                -20°C
Agarose                              1 gm                  RT
50X TAE                            7.5 ml                 4°C
PCR Tubes                        5 Nos.                 RT
Ethidium bromide (EtBr)   25ul                  RT