BioBharati provides modified cloned version of Pfu DNA Polymerase (originated from Pyrococcus furiosus) expressed in E. coli. Modified Pfu-based PCR reactions are for high fidelity DNA cloning, expression and mutagenesis.
Reagents supplied:
• Pfu Plus DNA Polymerase, (5 U/µl)
• 5 X Pfu Plus Reaction buffer

Storage Buffer: 20 mM Tris-HCl, pH 7.4; 100 mM NaCl; 0.1 mM EDTA; 1mM DTT; 200µg/ml BSA; 50% glycerol

Application: PCR (tested with up to 12 Kbp Genomic DNA templates)

Storage instructions: -20°C.