Introduction:
• Plasmids are self replicating, double stranded, circular DNA molecules that are maintained in bacteria as independent extra chromosomal entities.
• The size of plasmid ranges from 1 Kbp to 500 Kbp
Principle:
DNA isolation involves the following steps:
1. Bacterial cell harvesting & cell lysis
2. Denaturation of proteins & cell debris
3. Renaturation & precipitation of plasmid
4. Purification of plasmid