Restriction Digestion KIT
• A restriction enzyme or restriction endonuclease, or restrictase is an enzyme that cleaves double stranded DNA into fragments at or near specific recognition sites within that DNA molecules.
• These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses.
Over 3,000 restriction enzymes are now known and more than 600 of restriction enzymes are commercially available for routine use in laboratories as a vital tool in molecular cloning for DNA modifications.
Restriction enzymes are primarily used in the laboratory for generating 1) Sticky Ends and 2) Blunt Ends DNA molecules for cloning purposes.