SDS-PAGE CBB Staining Teaching Kit
> To learn the preparation of Sodium Dodecyl Sulphate Polyacrylamide Gel (SDS-PAGE)
> To analyze protein purity by SDS-PAGE
> To determine the molecular weight of pure protein by comparing with known protein marker
SDS PAGE: SDS-PAGE is the most widely used method and considered as gold standard for qualitatively analysing any protein sample within a mixture of other protein samples. From SDS PAGE one can check the protein purity and also determine the molecular weight of a protein by comparing with protein standard of known molecular weight. SDS or sodium dodecyl sulphate is an anionic detergent that binds strongly to proteins with a uniform negative charge, which nullify the basic charges present on that protein.
SDS binds fairly uniformly with protein in charge to mass ratio. Generally 1.4gm of SDS binds with 1 gm of protein. As a result the protein becomes fully negatively charged and they separate according to their molecular weight within a porous acrylamide bisacrylamide matrix. There will be no differential migration based on charge.
Cat. # BB-PATK010: BioBharati Kit is designed to carry out 10 SDS PAGE. In this kit, 3 unknown protein samples along with a known protein molecular weight marker are provided. These samples are ready to use. The amount of protein is sufficient enough so that students can load each sample in triplicate in a gel.
Sufficient staining and de-staining solution are provided to stain and de-stain 10 gels. Electrophoresis equipment and transfer apparatus should be arranged by user.
Duration of experiment: Duration of the experiment is around 6-8 hours.