Introduction: Our Streptavidin bound Agarose Beads are useful for assay development using affinity chromatography purifications of biotin tagged proteins or nucleic acid and immunoprecipitation.
Recombinant Streptavidin purified from E.coli is covalently bound to 6% cross-linked agarose bead. The Streptavidin-linked resin which is stable at pH 2-11 can be used to separate biotinylated products from non-biotinylated products. Biotinylated molecules can be RNA, DNA or protein which binds to the Streptavidin agarose bead with extremely high affinity. This high affinity interaction is useful to monitor protein-RNA, protein-DNA and protein-protein interaction.
Product Description: Approximately 1.5 mg/ml Streptavidin has been immobilized on 6% cross-linked beaded agarose. This product is supplied as 50% suspension of Streptavidin Resin in aqueous ethanol when packed.
BEAD GEOMETRY & SIZE : Spherical, ∼ 50 – 150 μm diameter
CROSSLINKED : Yes
BEAD CROSSLINKING % : 6%
ACTIVATING GROUP : Carbonyl
MATRIX STABILITY : Stable in all commonly used reagents
STORAGE SOLUTION : 20% aqueous ethanol
STORAGE TEMPERATURE : 4°C to 8°C. DO NOT FREEZE.
STABILITY : Stable for 1 year under proper storage condition