|Cat # BB-E0025||5,000 U (100 μl)|
T7 RNA PolymerasePDF DOWNLOAD
This is a DNA dependent RNA Polymerase, expressed in E.coli and purified. It synthesizes RNA in the 5’-3’ direction from a DNA containing T7 bacteriophage promoter. T7 RNA Polymerase is purified as a recombinant protein expressed in E. coli.
• T7 RNA Polymerase, (50U/µl)
• 10 X reaction Buffer (400 mM Tris pH 7.5, 60 mM MgCl2, 20 mM Spermidine HCl, 50 mM NaCl)
Storage Buffer: 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 50% Glycerol, 1 mM DTT, 0.1% Triton X-100.