Catalogue No. Quantity/Specifications
Cat # BB-E0025 5,000 U (100 μl)


Technology

T7 RNA Polymerase

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T7 RNA Polymerase

This is a DNA dependent RNA Polymerase, expressed in E.coli and purified. It synthesizes RNA in the 5’-3’ direction from a DNA containing T7 bacteriophage promoter. T7 RNA Polymerase is purified as a recombinant protein expressed in E. coli.
Reagents supplied:
• T7 RNA Polymerase, (50U/µl)
• 10 X reaction Buffer (400 mM Tris pH 7.5, 60 mM MgCl2, 20 mM Spermidine HCl, 50 mM NaCl)
Storage Buffer: 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 50% Glycerol, 1 mM DTT, 0.1% Triton X-100.
Storage: –20°C.