Catalogue No. Quantity/Specifications
Cat # BB-AB-E0011 250 U (100 μl) + 1ml dNTPmix (2.5 mM)


Technology

Taq DNA Polymerase KIT

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Taq DNA Polymerase KIT

Description: BioBharati provides cloned version of Taq DNA Polymerase which is expressed in E. coli. This recombinant Taq DNA Polymerase shows identical characteristics to native Taq from Thermus aquaticus with respect to activity, specificity, thermo stability and performance in PCR. Taq DNA Polymerase catalyzes the incorporation of dNTPs into DNA. It requires a DNA template, a primer terminus, and the divalent cation Mg . Taq DNA Polymerase contains a polymerization dependent 5′-3′ exonuclease activity. It does not have a 3′-5′ exonuclease and thus no proof reading function. Despite this, the enzyme synthesizes DNA in vitro with reasonable fidelity.
Reagents supplied:
Taq DNA Polymerase, 250 U(2.5 U/µl)
10X Taq Reaction buffer (100 mM Tris-HCl, pH 8.8, 500 mM KCl,
0.1% Tween 20,15 mM MgCl2),1.5 mL 2.5 mM dNTP (1ml)
Storage Buffer: 25 mM Sodium Phosphate, pH 8, 75 mM NaCl, 0.1 mM EDTA, 1mM DTT, 50% glycerol (v/v)
Application:PCR (tested with up to 10Kbp DNA template), RT-PCR.
Storage instructions: -20°C