Co-NTA Agarose Beads

Cat # BB-CA003A (5ml) 
Cat # BB-CA003B (10ml) 
Cat # BB-CA003C (25ml) 
Cat # BB-CA003D (50ml) 
Cat # BB-CA003E (100ml)

Bead size: Spherical, 50 – 150μm

Cross-Linked: Yes

Ligand: Nitilotriacetic acid (NTA)

Agarose %: 6%

Binding Capacity: ≥ 25mg 6xHis-tagged protein/ml Beads

Volume %: 50% (v/v) aqueous suspension with 20% Ethanol

Application: Gravity-based protein purification.

Introduction: Nitrilotriacetic acid (NTA)-crosslinked-agarose resin consists of NTA groups covalently immobilized on agarose beads by stable ether linkages via a spacer arm. NTA is a tetradentate chelating agent forming octahedral coordination complex with divalent transition metal cations like Co²⁺ along with two water molecules occupied in cis manner. This ready to use resin, after loading with Co²⁺ is ideal for rapid purifications of His-tagged proteins.

Storage Temperature: 4°C

Expiry: After18 months from receiving if proper storage condition is followed.