Co-NTA Agarose Beads
Cat # BB-CA003A (5ml)
Cat # BB-CA003B (10ml)
Cat # BB-CA003C (25ml)
Cat # BB-CA003D (50ml)
Cat # BB-CA003E (100ml)
Bead size: Spherical, 50 – 150μm
Ligand: Nitilotriacetic acid (NTA)
Agarose %: 6%
Binding Capacity: ≥ 25mg 6xHis-tagged protein/ml Beads
Volume %: 50% (v/v) aqueous suspension with 20% Ethanol
Application: Gravity-based protein purification.
Introduction: Nitrilotriacetic acid (NTA)-crosslinked-agarose resin consists of NTA groups covalently immobilized on agarose beads by stable ether linkages via a spacer arm. NTA is a tetradentate chelating agent forming octahedral coordination complex with divalent transition metal cations like Co2+ along with two water molecules occupied in cis manner. This ready to use resin, after loading with Co2+ is ideal for rapid purifications of His-tagged proteins.
Storage Temperature: 4°C
Expiry: After18 months from receiving if proper storage condition is followed.