Western Blot Teaching Kit Cat# BB-ITK050 (5 reactions) Aim: To learn the technique of Western Blotting in order to detect a specific protein in a blood or tissue sample with the help of that protein specific antibody. It involves the following experiments: Electrophoresis of the protein (SDS-PAGE). Transfer of protein onto a […]
Rocket Immunoelectrophoresis Teaching Kit Cat# BB-ITK040 (10 Reactions) Objective: To perform Rocket Immuno-electrophoresis (RIEP) for the determination of various concentrations of antigen in given unknown sample. Principle: Rocket Immunoelectrophoresis (RIEP) also known, as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample. Various
Immunoelectrophoresis Teaching Kit Cat# BB-ITK030 (10 Reactions) Objectives: To learn the technique of immunoelectrophoresis. Principle: Immunoelectrophoresis is a powerful technique to characterize antibodies. This technique is based on the principle of electrophoresis of antigen for immunodiffusion with a poly-specific antiserum to form precipitin bands. Electrophoresis: During electrophoresis, molecules placed in an electric field acquire
Ouchterlony Double Diffusion Teaching Kit Cat# BB-ITK020 (10 Reactions) Objective: To study the reaction pattern of an antigen with a set of antibodies by Ouchterlony Double Diffusion method. Principle: Interaction between antigen (Ag) and antibody (Ab) at the molecular level forms the basis for several techniques that are useful in modern day scientific studies and
Human OGG1 Cat# BB-PE0245 100µg (1mg/ml) Product type: Human OGG1 Protein. Source: Recombinant His Tag protein expressed in E. coli Alternative Name: HMMH, HOGG1, MUTM, OGH1 Purity: >95%, by SDS-PAGE under reducing conditions and visualized by coomassie stain. Protein Sequence: HHHHHHSSGLVPRGSHTGSMPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNK ELGNFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG Formulation: Supplied as a 0.2 μm filtered solution in PBS at 100 μg (1mg/ml)
Mouse-TNFα Cat# BB-PE0175 100µg (1mg/ml) Product type: Murine TNFα extracellular domain Source: Recombinant protein expressed in E. coli Other Name: TNFSF1A, TNFSF2 Protein Sequence: LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL Purity: Greater than 98% Description: Tumor necrosis factor alpha (TNF-α) is a member of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis,
Human TNF α Cat# BB-PE0170 100µg (1mg/ml) Product type: Human tumor necrosis factor alpha (TNFα) extracellular domain Source: Recombinant protein expressed in E. coli Other Name: TNFSF1A, TNFSF2 Protein Sequence: MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREESPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL Purity: Greater than 98% evaluated based on SDS-PAGE Formulation: Supplied as a 0.2 μm filtered solution in PBS at 100 μg (1mg/ml) containing 50%
Mouse-IL-1β Cat# BB-PE0165 100µg (1mg/ml) Product type: Murine interleukin1beta (IL-1β) Extracellular domain (aa117-269) Source: Recombinant protein expressed in E. coli Protein Sequence: VPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS Purity: >98%, by SDS-PAGE under reducing conditions and visualized by coomassie stain Formulation: Supplied as a 0.2 μm filtered solution in PBS at 100 μg (1mg/ml) containing 50% Glycerol. Specificity: Acts both on
Rabbit anti-Rat IgG-HRP Cat# BB-SAB03A (250µ) Cat# BB-SAB03B (500µl) Cat# BB-SAB03C (1000µl) Clonality: Polyclonal Host: Rabbit Immunogen: Rat IgG (H+L chain) Conjugate: Horseradish peroxidase (HRP) Purification: Affinity chromatography. Concentration: 1mg/ml Application: Western blotting, ELISA. Recommended Dilution: Western Blotting: 1:3,000-1:5,000 ELISA: 1:10,000-1:20,000 Storage buffer: Sodium azide free 100mM phosphate buffered saline pH-7.3, containing 50% Glycerol and 1.5% bovine
Rabbit anti-Mouse IgG-HRP Cat# BB-SAB02A (250µl) Cat# BB-SAB02B (500µl) Cat# BB-SAB02C (1000µl) Clonality: Polyclonal Host: Rabbit Immunogen: Mouse IgG (H+L chain) Conjugate: Horseradish peroxidase (HRP) Purification: Affinity chromatography. Concentration: 1mg/ml Application: Western blotting, ELISA. Recommended Dilution: Western Blotting: 1:3,000-1:5,000 ELISA: 1:10,000-1:20,000 Storage buffer: Sodium azide free 100mM phosphate
