Agarose Gel Electrophoresis Cat# BB-MTK060 (10 reactions) Aim: To visualize the separation of different DNA fragments (provided in the kit) of different molecular weights by using agarose gel electrophoresis. Principle: Agarose Gel Electrophoresis is a method to separate nucleic acid (DNA or RNA) in agarose matrix. It can be used to separate mixed …
Restriction Digestion Cat# BB-MTK040S (5 reactions) Cat# BB-MTK040 (10 reactions) Principle: A restriction enzyme or restriction endonuclease, or restrictase is an enzyme that cleaves double stranded DNA into fragments at or near specific recognition sites within that DNA molecule. These enzymes are found in bacteria and archaea and provide defense mechanism against invading viruses. Over …
Bacterial Transformation Cat# BB-MTK030S (5reactions) Cat# BB-MTK030 (10 reactions) Background and principle: By definition transformation is a process of genetic alteration in a bacterium associated with an uptake of a naked exogenous DNA. To uptake a naked DNA a bacterium must be in a special state called ‘competent’. Usually a bacterium is …
Plasmid DNA isolation Cat# BB-MTK020S (5 reactions) Cat# BB-MTK020 (10 reactions) Introduction: Plasmids are self-replicating, double stranded, circular DNA molecules that are maintained in bacteria as independent extra chromosomal entities. The size of plasmid ranges from 1 Kbp to 500 Kbp Principle: Bacterial host grow in LB broth medium at recommended …
PCR Teaching kit Cat# BB-MTK010S (5Reactions) Cat# BB-MTK010 (10Reactions) Objectives: To perform PCR amplification of specific target sequence from template To analyze the amplified product by Agarose gel Principle: PCR (Polymerase Chain Reaction) is an in vitro method of enzymatic synthesis of specific DNA sequence, developed by Kary Mullis in 1983. It …
Radial Immunodiffusion Cat No# BB-ITK010 (10 Reactions) Objective: To study the immunodiffusion technique by Single Radial Immunodiffusion. Principle: Single Radial Immunodiffusion (RID) is used extensively for the quantitative estimation of antigens. The antigen antibody precipitation is made more sensitive by the incorporation of antiserum in the agarose. Antigen (Ag) is then …
Thin Layer Chromatography Cat# BB-C030 (10 reactions) Aim: To separate a mixture of amino acids by Thin Layer Chromatography (TLC) and identifying the unknown amino acids in comparison with known amino acids by measuring their Rf values. Principle: Thin Layer Chromatography (TLC) is a sensitive, fast, simple and in-expensive analytical technique. It …
DOT ELISA TEACHING KIT Cat# BB-ITK110 (10 Reactions) Aim: To learn the technique of Dot ELISA to detect a specific protein with the help of that protein specific antibody. It involves the following experiments: Coating of the antigen on Dot ELISA Strips. Blocking & Incubation with Primary and Secondary Antibody. Detection Principle: …
Lymphocyte Separation Teaching Kit Cat# BB-ITK100, Pack Size: 5 reactions Aim: To separate lymphocyte from whole blood by Density Gradient Centrifugation method. Principle: A lymphocyte is a small white blood cell, usually 7-8µm in length, present in vertebrate’s immune system. Three types of lymphocytes are T cells, B cells and NK cells which …
Antibody Capture ELISA Teaching Kit Cat# BB-ITK060 (4 Reactions) Aim: To determine the antibody concentration by Antibody Captured ELISA. It involves the following experiments: Coating the wells with Blocking & Incubation with Primary and Secondary Detection. Principle: Enzyme-linked immunosorbent assay, commonly known as ELISA is a popular format of analytical biochemistry assay that …
