pBBL-eGFP Vector Cat# BB-V0080 (10µg) Description: pBBL-eGFP is a shuttle vector used to express proteins in mammalian cells as eGFP-fusion proteins. The plasmid is amplified in E.coli. Kanamycin and neomycin are the selective markers for the vector. Store at -20°C. Download PDF Address EN-35, First floor, Salt Lake, Sector – V, Kolkata – 700091, …
TEV protease Cat# BB-E0030 (0.25mg) Description: This cysteine protease, which originates from the Tobacco Etche Virus (TEV) is highly site specific and is expressed in E. coli. It recognizes the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) (ENLYFQ (G/S)) and cleaves between the Gln and Gly/Ser residues. Although the optimal temperature for activity is 30o C, it can work over …
T7 RNA Polymerase Cat # BB-E0025 (5000U) Description: This is a DNA dependent RNA Polymerase. It synthesizes RNA in the 5’-3’ direction from a DNA containing the T7 bacteriophage promoter. Reagents supplied: T7 RNA Polymerase, (50U/µl) 10X T7 reaction Buffer (10X Transcription Buffer) (400 mM Tris pH 7.5, 60 mM MgCl2, 20 mM Spermidine …
RNase Inhibitor Cat # BB-X0010 (2000 U) Description: RNase inhibitor inhibits the activity of RNase A, B and C by noncovalently binding them in 1:1 ratio. It does not inhibit other eukaryotic RNases or the prokaryotic RNases I and H. This murine RNase Inhibitor is purified as a recombinant protein expressed in E. coli. …
Super Reverse Transcriptase MuLV Cat# BB-E0042 (20000 U) Description: Murine Leukemia Virus Reverse Transcriptase (MuLV RT) is an RNA-dependent DNA polymerase used for cDNA synthesis. Reagents supplied: Super RT (200 U/µl) , 100 µl 5x RT Buffer (250 mM Tris-HCl, pH 4; 375 mM KCl; 15 mM MgCl2), 400 µl Storage Buffer: 20mM Tris-HCl …
Super Reverse Transcriptase MuLVEasy Kit Cat# BB-E0045 (50 reactions) Description: Murine Leukemia Virus Reverse Transcriptase (MuLV RT) is an RNA-dependent DNA polymerase used for cDNA synthesis. Reagents supplied: Super RTEasy Enzyme mix: 100 µl 2x Reaction Mix (contains dNTPs, Oligo dT and Random Hexamer): 500 µl Nuclease free water: 500 µl This Super RTEasy …
Super Reverse Transcriptase MuLV Kit Cat# BB-E0043 (10000 U) Description: Murine Leukemia Virus Reverse Transcriptase (MuLV RT) is an RNA-dependent DNA polymerase used for cDNA synthesis. Reagents supplied: Super RT (200 U/µl) , 50 µl 5x RT Buffer (250 mM Tris-HCl, pH 4; 375 mM KCl; 15 mM MgCl2), 200 µl dNTP mix (10mM), …
Pfu Plus DNA Polymerase Cat # BB-E0021S (100 U) Cat # BB-E0021 (250 U) Description: This cloned version of Pfu DNA Polymerase originates from Pyrococcus furiosus. Pfu-based PCR reactions are for high fidelity DNA cloning, expression and mutagenesis. Reagents supplied: Pfu Plus DNA Polymerase, (5 U/µl) 5X Pfu Plus Reaction buffer Storage Buffer: 20 mM …
Taq DNA Polymerase KIT Cat # BB-E0011 (500 U) Description: BioBharati provides a cloned version of Taq DNA Polymerase, which is expressed in E. Coli. This recombinant Taq DNA Polymerase is identical to native Taq from Thermus aquaticus with respect to activity, specificity, thermostability and performance in PCR. Taq DNA Polymerase catalyzes the incorporation …
Taq DNA Polymerase Cat # BB-E0010S (250 U) Cat # BB-E0010 (1000 U) Description: BioBharati provides a cloned version of Taq DNA Polymerase, which is expressed in E. Coli. This recombinant Taq DNA Polymerase is similar to native Taq from Thermus aquaticus with respect to activity, specificity, thermostability and performance in PCR. Taq DNA Polymerase …
